cd68 polyclonal rabbit anti human antibody  (Proteintech)

Journal: Frontiers in Cell and Developmental Biology

Article Title: Syndecan-1 as a predictor of vulnerable atherosclerotic plaques

doi: 10.3389/fcell.2024.1415788

Figure Lengend Snippet: Analysis of destabilizing components in atherosclerotic plaque. (A) The expression of lipid deposition in aortic root plaques was detected by oil red O with a scale bar of 100 μm, immunofluorescence detection of CD68 expression (macrophage marker) and VEGF in aortic root plaques with a scale bar of 20 μm, and IHC detection of MMP-9 expression in aortic root plaques with a scale bar of 100 μm. (B–E) Statistical chart for quantitative analysis of oil red o staining, CD68, VEGF and MMP-9 in the aortic root plaques (n = 6, one-way ANOVA). (F) Immunofluorescence detection of BODIPY and VEGF expression in LCCA plaques with a scale bar of 100 μm. IHC detection of CD68 and MMP-9 expression in LLCCA plaques with a scale bar of 100 μm. (G–J) Statistical chart for quantitative analysis of BODIPY staining, VEGF, CD68 and MMP-9 in the LCCA plaques (n = 6, unpaired t -test). * P < 0.05, ** P < 0.01, *** P < 0.001, compared with CD group; # P < 0.05, ## P < 0.01, ### P < 0.001, compared with CD + PLCA group; && P < 0.01, &&& P < 0.001, compared to HFD group.

Article Snippet: After blocking, sections were incubated with a rabbit anti-human CD68 (1:100, bs-4819R, BIOSS, China), α-SMA (1:100, A1011, Abclonal, China) and mouse anti-human VEGF (1:100, TA500289, Origene, United States of America) antibodies overnight at 4°C.

Techniques: Expressing, Immunofluorescence, Marker, Staining

Journal: Cell

Article Title: In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies

doi: 10.1016/j.cell.2021.06.021

Figure Lengend Snippet: Lung histopathology of Ab-treated and SARS-CoV-2 challenged cynomolgus macaques, related to and (A) Representative images of hematoxylin and eosin (H&E) staining and SARS-CoV-2 antigen immunohistochemistry (IHC) staining from each group. All images were taken at 10x magnification. The images in this figure are representative of the average severity of pathologic processes observed and recorded during microscopic evaluation. Red arrows indicate SARS-CoV-2 infection foci. (B) Following microscopic evaluation of DH1052, 1 animal (BB536A) out of 5 animals in this group exhibited histologic features that was substantially more severe than the rest of the cohort and may suggest some degree of Ab-mediated disease enhancement. The features were characterized by prominent perivascular mononuclear inflammation ( ∗ ) and a substantial amount of perivascular and alveolar edema (fluid; X). These findings suggest a vaso-centric process with some degree of altered vascular permeability. The remaining 4 animals in DH1052 group had inflammatory changes that ranged from minimal to moderate severity and more infiltrates were mixed and predominantly polymorphonuclear with lesser mononuclear cell involvement and present in the alveolar spaces. (C-E) Expression of macrophage activation markers in macaque lung tissues. An animal from the CH65 control group (C), the DH1052-treated animal (BB536A) that exhibited substantially more severe lung inflammation (D), and an animal from the NTD NAb DH1050.1 group (E) were selected for Immunohistochemistry (IHC) staining. Immunohistochemical staining was performed using MHCII, CD68, IBA1 and CD163 to detect classically activated macrophages (M1) and/or alternatively activated macrophages (M2). CD11b is a macrophage/monocyte marker and CD3 is a T cell marker. All images are 10x magnification; scale bars = 100μm.

Article Snippet: Rabbit anti-human CD68 polyclonal Ab , Sigma-Millipore , Cat# HPA048982, RRID: AB_2680587.

Techniques: Histopathology, Staining, Immunohistochemistry, Infection, Permeability, Expressing, Activation Assay, Control, Immunohistochemical staining, Marker

Journal: Cell

Article Title: In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies

doi: 10.1016/j.cell.2021.06.021

Figure Lengend Snippet:

Article Snippet: Rabbit anti-human CD68 polyclonal Ab , Sigma-Millipore , Cat# HPA048982, RRID: AB_2680587.

Techniques: Virus, Clinical Proteomics, Recombinant, Staining, Reverse Transcription, Random Hexamer, Luciferase, Cell Culture, Lysis, Membrane, Multiplex Assay, Reporter Gene Assay, Binding Assay, Expressing, Transgenic Assay, Sequencing, Software